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Growth Hormone Receptor Upregulation The peptide increases the expression of growth hormone receptors on cell surfaces
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Generation of HAP1 knockout cell line For human HMBS knockout, we chose a sgRNA targeting an early coding region of the HMBS gene (5-GCAGCTTGCTCGCATACAGA-3) and cloned this guide into the pCRISPR_v3.1 plasmid
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Heffernan 2001b This metabolic selectivity has a dosage implication: once the beta-3 receptors in adipose tissue are adequately occupied, additional peptide does not proportionally increase the lipolytic signal
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